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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
83
- 克隆性:
单克隆
- 保质期:
1年
- 抗体英文名:
GPX3
- 抗体名:
谷胱甘肽过氧化酶3
- 适应物种:
人/动物/植物
- 应用范围:
WB,ELISA等
- 浓度:
1mg/ml
- 保存条件:
-20 °
- 规格:
100ul/200ul
| 规格: | 100ul | 产品价格: | ¥1580.0 |
|---|---|---|---|
| 规格: | 200ul | 产品价格: | ¥2480.0 |
英文名称 : GPX3
中文名称 : 谷胱甘肽过氧化酶3
别 名 : Extracellular glutathione peroxidase; Glutathione peroxidase 3 (plasma); Glutathione peroxidase 3; GPX 3; GPx P; GPx-3; GPx-P; GPX3; GPX3_HUMAN; GPXP; GSHPx 3; GSHPx P; GSHPx-3; GSHPx-P; Plasma glutathione peroxidase 3; Plasma glutathione peroxidase.
研究领域 : 细胞生物 信号转导 新陈代谢 线粒体
抗体来源 : Rabbit
克隆类型 : Polyclonal
交叉反应 : Human, Mouse, Rat, Pig, Cow,
产品应用 : ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 : 23kDa
细胞定位 : 分泌型蛋白 线粒体
性 状 : Lyophilized or Liquid
浓 度 : 1mg/ml
免 疫 原 : KLH conjugated synthetic peptide derived from human GPX3:1-100/226
亚 型 : IgG
纯化方法 : affinity purified by Protein A
储 存 液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed : PubMed
产品介绍 : Glutathione peroxidases(Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organichydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPxfamily to be discovered. GPx2, also known as gastrointestinal GPx(GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract.Extracellular plasma GPx(pGPxor GPx3) is mainly expressed by the kidney from where it is released into the blood. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxylipids in low density lipoprotein orthymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx(eGPxor GPx5)isoforms, possess selenocysteine at the active site.
Function:
Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.
Subunit:
Homotetramer.
Subcellular Location:
Secreted.
Tissue Specificity:
Secreted in plasma.
Post-translational modifications:
The N-terminus is blocked.
Similarity:
Belongs to the glutathione peroxidase family.
SWISS:
P22352
Gene ID:
2878
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
谷胱甘肽过氧化物酶(GSH-Px)是机体内广泛存在的一种重要的过氧化物分解酶。硒是GSH-Px酶系的组成成分,它能催化GSH变为GSSG,使有毒的过氧化物还原成无毒的羟基化合物,同时促进H2O2的分解,从而保护细胞膜的结构及功能不受过氧化物的干扰及损害。GSH-Px的活性中心是硒半胱氨酸,其活力大小可以反映机体硒水平。GSH-Px酶系主要包括4种不同的GSH-Px,分别为胞浆GSH-Px、血浆GSH-Px、磷脂氢过氧化物GSH-Px及胃肠道专属性GSH-Px。胞浆GSH-Px由4个相同的分子量大小为22kDa的亚基构成四聚体,每个亚基含有1个分子硒半胱氨酸,广泛存在于机体内各个组织,以肝脏红细胞为多。它的生理功能主要是催化GSH参与过氧化反应,清除在细胞呼吸代谢过程中产生的过氧化物和羟自由基,从而减轻细胞膜多不饱和脂肪酸的过氧化作用
产品图片
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文献和实验C10 H17 N3 SO6 。为γ -L-谷氨酰 -L-半胱氨酞甘氨酸( 1)。谷胱甘肽如( 2)所示在活体内参与氧化还原反应。所以具有保护如组织蛋白酶、番木瓜酶及琥珀酸脱氢酶那样具有 SH基酶的 SH基作用。另外是催化甲基乙二醛形成乳酸的乳酰谷胱甘肽裂解酶( EC. 4. 4. 1. 5)不可缺少的辅助基质。在解毒作用中形成硫醚氨酸,被认为有毒物质先与谷胱甘肽结合使谷氨酸与甘氨酸部分切断。
谷胱甘肽是属于含有巯基的、小分子肽类物质,具有两种重要的抗氧化作用和整合解毒作用。谷胱甘肽是由谷氨酸、半胱氨酸和甘氨酸结合而成的三肽,半胱氨酸上的巯基为其活性基团(故谷胱甘肽常简写为G-SH),易于碘乙酸、芥子气(一种毒气)、铅、汞、砷等重金属盐络合,而具有了整合解毒作用。谷胱甘肽(尤其是肝细胞内的谷胱甘肽)具有非常重要的生理作用就是整合解毒作用,能与某些药物(如扑热息痛)、毒素(如自由基、重金属)等结合,参与生物转化作用,从而把机体内有害的毒物转化为无害的物质,排泄出体外。
(1)帮助鉴别急性白血病的类型:①急性粒细胞白血病时,白血病性原始粒细胞可呈阳性反应,阳性颗粒一般较多,较粗大,常呈局限性分布;急性淋巴细胞白血病时,原始淋巴细胞和幼淋巴细胞均呈阴性反应;急性单核细胞白血病时,白血病性原始单核细胞呈阴性反应,有时虽少数可呈弱阳性反应,但阳性颗粒少而细小,常弥散分布。医学教育|网搜集整理②小型原始粒细胞和原始淋巴细胞不易区别,如果小型原始细胞呈过氧化物酶阳性反应,可确定为小型原始粒细胞。③急性早幼粒细胞白血病有时须与急性单核细胞白血病鉴别,如果白血病细胞呈










