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- PVT17118
- 2025年08月25日
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- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pMD19T-Ska2-m
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pMD19T-Ska2-m/ pMD19T-Ska2-m璐ㄧ矑
pMD19T-Ska2-m Plasmid
PVT17118 2ug
pMD19T-Ska2-m Plasmid Alternative Name
NM_025377.3,1110001A07Rik, C78640, Fam33a
pMD19T-Ska2-m PlasmidInformation
Promoter
Prokaryotic resistance Amp
Screening marker
Clone strain DH5a
Culture Conditions LB/37 Celsius degree
Forward primer M13F
Reverse primer M13R
pMD19T-Ska2-m Plasmid Description
pMD19T-Ska2-m Plasmid is a DNA cloning plasmid
NOTE: For research use only!
3128136151
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文献和实验This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
Purification of Plasmid from 50 ml-culture
amount of phenol dissolved in the solution. Take aquaous phase (or remove organic phase). 19. Add 0.1 volume of 3 M sodium acetate and 3 volume of ethanol into the plasmid solution. Spin at 14,000 rpm for 5 min at 4 C. Discard the supernatant. Rinse
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