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- PVT16507
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pCMV-SPORT6-WNT2(NM_003391.2)
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pCMV-SPORT6-WNT2(NM_003391.2)/ pCMV-SPORT6-WNT2(NM_003391.2)璐ㄧ矑
pCMV-SPORT6-WNT2(NM_003391.2) Plasmid
PVT16507 2ug
pCMV-SPORT6-WNT2(NM_003391.2) Plasmid Alternative Name
NM_003391.2,INT1L1, IRP
pCMV-SPORT6-WNT2(NM_003391.2) PlasmidInformation
Promoter
Prokaryotic resistance Amp
Screening marker
Clone strain DH5a
Culture Conditions LB/37 Celsius degree
Forward primer SP6
Reverse primer T7
pCMV-SPORT6-WNT2(NM_003391.2) Plasmid Description
pCMV-SPORT6-WNT2(NM_003391.2) Plasmid is a DNA cloning plasmid
NOTE: For research use only!
3128136151
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文献和实验cell pellet in 100 μl of GTE buffer (50 mM Glucose, 25 mM Tris-Cl, 10 mM EDTA, pH 8). Vortex gently if necessary. 4. Add 200 μl of NaOH/SDS lysis solution (0.2 M NaOH, 1% SDS). Invert tube 6-8 times. 5. IMMEDIATELY add 150 μl
Plasmid DNA Isolation from Bacteria
Materials: TE buffer 10% (w/v) sodium dodecyl sulfate (SDS) 20 mg/ml proteinase K phenol\chloroform (50:50) isopropanol 70% ethanol 3M sodium acetate pH 5.2 Phase Lock GelTM (5 Prime, 3 Prime, Inc) 1.Grow E. coli culture overnight in rich
and long term storage. 17. Measure the concentration of the plasmid DNA by diluting stock into water at 1:200 or 5 m l of DNA per 1 ml of water (blank spectrophotometer to water). The absorbance at 260 nm multiplied by ten is the concentration
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