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- LSM BIO
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- PVT15922
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pCMV-FLAG-Nsd2-m(REIIBP)
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pCMV-FLAG-Nsd2-m(REIIBP)/ pCMV-FLAG-Nsd2-m(REIIBP)璐ㄧ矑
pCMV-FLAG-Nsd2-m(REIIBP) Plasmid
PVT15922 2ug
pCMV-FLAG-Nsd2-m(REIIBP) Plasmid Alternative Name
pCMV-FLAG-Nsd2-m(REIIBP) PlasmidInformation
Promoter CMV
Prokaryotic resistance Amp
Screening marker
Clone strain DH5a
Culture Conditions LB/37 Celsius degree
Forward primer CMV-F
Reverse primer
pCMV-FLAG-Nsd2-m(REIIBP) Plasmid Description
pCMV-FLAG-Nsd2-m(REIIBP) Plasmid is a ORF expression plasmid
NOTE: For research use only!
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文献和实验of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
the more cells to begin with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use
Summary WCE Extracts are prepared from strain SHY282 (TFC4-Flag-EE (HpaI)).TFIIIC complex is first purified by flag affinity: TFC4 contains a single copy of the flag epitope.The flag purified TFIIIC is then further purified by anion exchange
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