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- LSM BIO
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- PVT15908
- 2025年07月07日
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- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pSG5 Large T
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pSG5 Large T/ pSG5 Large T璐ㄧ矑
pSG5 Large T Plasmid
PVT15908 2ug
pSG5 Large T Plasmid Alternative Name
pSG5 Large T PlasmidInformation
Promoter
Prokaryotic resistance Amp
Screening marker
Clone strain DH5a
Culture Conditions LB/37 Celsius degree
Forward primer
Reverse primer
pSG5 Large T Plasmid Description
pSG5 Large T Plasmid is a ORF expression plasmid
NOTE: For research use only!
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文献和实验Large-Scale Isolation of Plasmid DNA
实验步骤 1. Inoculate 200 ml of L2 containing appropriate selective agent with a single colony or culture containing the desired plasmid. Grow at 37 degrees overnight. 2. Spin down cells at 5000 rpm for 15 minutes
Large-Scale Isolation of Ti Plasmid DNA
) and TR-(8 kb) of the bipartite octopine type T-DNA (4.5; see Fig.1 ). Other important features of the Ti plasmids include; the large virulence region, essential for transfer of the T-DNA; the ori region, responsible for the Ti plasmid replication
Large Scale Plasmid Preps: PEG method
Sorval tubes (max 20 mL/tube) Add an equal volume of isopropanol to each tube. Mix. Spin 15 min. at 8,000 rpm (12,000g) in a JA-10 rotor. 8. Discard supernatant. Air dry pellet. Resuspend pellets in a total of 4 mL of T.E. with 100 µg/mL
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