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- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pLgw V5-EcoDam
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pLgw V5-EcoDam/ pLgw V5-EcoDam璐ㄧ矑
pLgw V5-EcoDam
PVT14498 2ug
pLgw V5-EcoDam Search name
pLgw V5-EcoDam,pLgw V5-EcoDam vector,pLgw V5-EcoDam plasmid,pLgw V5-EcoDam map
pLgw V5-EcoDam Information
Bacterial Resistance(s): Amp
Screening:
Growth Strain(s): Stbl3
Growth Condition: LB/37 Centigrade
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文献和实验Inhibition of Glycogen Synthase Kinase-3 Methods in Molecular Biology v468. chapter 5 Abstract There are two homologous forms of glycogen synthase kinase (GSK)-3, GSK-3a and GSK-3b, whichplay overlapping roles in the regulation of Wnt
Materials Solution II: 0.2N NaOH/1% SDS Solution III: 3 M KOAc, pH4.8 RNAseA (DNAse free) 10 µg/mL Chloroform/Isoamyl alcohol (1/25 v/v) Isopropanol 70 % ethanol 13% PEG8000 Procedure 1) Culture 5 mL
Plasmid DNA Isolation from Bacteria
Materials: TE buffer 10% (w/v) sodium dodecyl sulfate (SDS) 20 mg/ml proteinase K phenol\chloroform (50:50) isopropanol 70% ethanol 3M sodium acetate pH 5.2 Phase Lock GelTM (5 Prime, 3 Prime, Inc) 1.Grow E. coli culture overnight in rich
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