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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pCR4-TOPO-SLC22A10(134-951bp)
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pCR4-TOPO-SLC22A10(134-951bp)/ pCR4-TOPO-SLC22A10(134-951bp)璐ㄧ矑
pCR4-TOPO-SLC22A10(134-951bp)
PVT14399 2ug
pCR4-TOPO-SLC22A10(134-951bp) Search name
pCR4-TOPO-SLC22A10(134-951bp),pCR4-TOPO-SLC22A10(134-951bp) vector,pCR4-TOPO-SLC22A10(134-951bp) plasmid,pCR4-TOPO-SLC22A10(134-951bp) map
pCR4-TOPO-SLC22A10(134-951bp) Information
Bacterial Resistance(s): Amp
Screening:
Growth Strain(s): DH5 alpha
Growth Condition: LB/37 Centigrade
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文献和实验Cloning of Taq polymerase-amplified PCR products
实验原理 The plasmid vector (pCR®II-TOPO® or pCR®2.1-TOPO®) is supplied linearized with: Single 3´-thymidine (T) overhangs for TA Cloning® Topoisomerase I covalently bound to the vector (referred to as "activated
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
and STA-PCR protocols. B112, B68, and B75 are BXH-2-derived leukemia samples. NC: healthy BXH-2 mice tail DNA used as a control. M: 100-bp DNA marker. The whitearrows indicate the differential bands. The size of DNA marker in base-pair is noted tothe left
From Genomes to Vaccines for Leishmaniasis
One hundred unique, randomly selected amastigote-expressed genes were PCR amplified to exclude the 5′-spliced leader, and the full-length genes subcloned into the TOPO-TA cloning vector. The genes were sequence-verified, excised using restriction
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