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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pET His6 Sumo TEV LIC cloning (2S-T)
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pET His6 Sumo TEV LIC cloning (2S-T)/ pET His6 Sumo TEV LIC cloning (2S-T)璐ㄧ矑
pET His6 Sumo TEV LIC cloning vector (2S-T)
PVT14035 2ug
pET His6 Sumo TEV LIC cloning vector (2S-T) Search name
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pET His6 Sumo TEV LIC cloning vector (2S-T) Information
Bacterial Resistance(s): Amp
Screening:
Growth Strain(s): DH5 alpha
Growth Condition: LB/37 Centigrade
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文献和实验to other types of fusion proteins. Expression of the fusion proteins Structures of the plasmid constructs encoding the IPIII-His and MBP fusion proteins are diagrammed in the figure; in both cases,the vector backbone is pET 29a(+)(Novagen Inc.,Madison,WI
Expression and Purification of Recombinant Proteins Using the pET System
The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in Escherichia coli . Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally
in vitro (2).Depending on the size of the DNA to be cloned, DNA fragments are inserted into plasmid (~0.1-10kb), lambda phage (~0-23kb), or cosmid (~35-50kb) vectors. Common cloning vectors such as the pGEM® series(d), pBR322, pUC/M13, pSP series(d), pET
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