pET-46 EK/LIC

pET-46 EK/LIC

pET46 EK/LIC载体基本信息

出品公司:	Ybscience
别名:	pET46 EK/LIC, pET 46 EK/LIC
质粒类型:	大肠杆菌蛋白表达
表达水平:	高
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5200 bp
5' 测序引物:	T7
5' 测序引物序列:	T7: 5'-TAATACGACTCACTATAGGG-3'
3' 测序引物:	T7t
3' 测序引物序列:	T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'
载体标签:	N-His, N-EK, C-S
载体抗性:	Ampicillin
表达宿主菌:	BL21(DE3) 、 Rosetta2(DE3)、C43(DE3)
备注:	For directional cloning of PCR-amplified DNA; ligation independent cloning; enterokinase cleavage site.
稳定性:	瞬时表达 Transient
组成型:	组成型 Constitutive
病毒/非病毒:	非病毒

pET46 EK/LIC载体质粒图谱和多克隆位点信息

pET46 EK/LIC载体简介

 

The pET-46 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for highlevel expression of polypeptides. Using specifically designed primers for amplification and the pET-46 Ek/LIC Cloning Kit (Cat. No. 71335-3), inserts can be efficiently cloned without the need for restriction digestion or ligation. Fusion proteins contain an N-terminal cleavable His•Tag® sequence. Fusion to an optional C-terminal S•TagTM sequence can also be created for detection, purification and quantification of fusion proteins. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 Terminator Primer (Cat. No. 69337-3). 

 

 

pET46 EK/LIC载体序列

LOCUS       pET-46 Ek/LIC    5200 bp     DNA  Circular   SYN
DEFINITION  pET-46 Ek/LIC
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="pET-46 Ek/LIC"
                     /mol_type="other DNA"
     gene            complement(582..1442)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(582..1442)
                     /label="ORF frame 3"
     promoter        complement(1485..1513)
                     /label="AmpR_promoter"
     rep_origin      1600..2219
                     /label="pBR322_origin"
     misc_feature    2618..2640
                     /label="pGEX_3_primer"
     misc_feature    complement(2634..2825)
                     /label="ROP"
     misc_feature    complement(3397..4488)
                     /label="lacI"
     CDS             complement(3397..4356)
                     /label="ORF frame 2"
     CDS             complement(4229..4729)
                     /label="ORF frame 1"
     gene            complement(4580..4831)
                     /label="tet (887 - 636)"
                     /gene="tet (887 - 636)"
     misc_feature    4763..4782
                     /label="pBRrevBam_primer"
     promoter        4870..4888
                     /label="T7_promoter"
     misc_feature    4888..4915
                     /label="lacO"
     misc_feature    4933..4949
                     /label="T7_transl_en_RBS"
     misc_feature    4984..4998
                     /label="EK"
     misc_feature    complement(5114..5132)
                     /label="T7_Terminal_primer"
     terminator      5133..5168
                     /label="T7_terminator"
ORIGIN
    1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
   61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
  121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
  181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
  241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
  301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
  361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
  421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat
  481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
  541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
  601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
  661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
  721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
  781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
  841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
  901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
  961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
 1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
 1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
 1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
 1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
 1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
 1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
 1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
 1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg
 1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac
 1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
 1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
 1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
 1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
 1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
 1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
 1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
 1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
 2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
 2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
 2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
 2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
 2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
 2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
 2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac
 2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
 2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
 2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
 2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
 2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
 2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
 2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
 2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
 2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt
 3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct
 3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac
 3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
 3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
 3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc
 3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
 3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
 3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
 3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
 3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
 3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
 3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
 3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
 3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
 3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
 3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
 3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
 4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
 4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
 4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
 4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
 4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
 4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
 4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
 4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
 4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
 4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
 4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
 4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
 4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
 4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc
 4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
 4921 aaataatttt gtttaacttt aagaaggaga tataccatgg cacatcacca ccaccatcac
 4981 gtggatgacg acgacaagag tccgggcttc tcctcaaatc tcgagtctgg taaagaaacc
 5041 gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagcgcagc ttaattaacc
 5101 taggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
 5161 ttgaggggtt ttttgctgaa aggaggaact atatccggat
//