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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pCDNA3.1-MCL1
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pCDNA3.1-MCL1/ pCDNA3.1-MCL1璐ㄧ矑
pCDNA3.1-MCL1
PVT14022 2ug
pCDNA3.1-MCL1 Search name
pCDNA3.1-MCL1,pCDNA3.1-MCL1 vector,pCDNA3.1-MCL1 plasmid,pCDNA3.1-MCL1 map
pCDNA3.1-MCL1 Information
Bacterial Resistance(s): Amp
Screening: G418
Growth Strain(s): DH5 alpha
Growth Condition: LB/37 Centigrade
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文献和实验wangch84 各位高手,兄弟最近忙于质粒构建,意图将一段长约4000 bp的片段,通过BamH1和EcoR1两个酶切位点连接到pcDNA3.1+载体上。情况如下: 1. 通过LA Taq酶扩增,并纯化回收目的片段(引物中带有酶切位点,保护性碱基=3,上游带有Kozak序列);由于回收效率很高,所以在进行下一步双酶切时只加了12 μl的目的片段,H2O 20 μl,10 X K BUFFER 4 μl,两种酶各2 μl,37℃作用过夜(12-14 h
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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