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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
Flag-SIRT1
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
Flag-SIRT1/ Flag-SIRT1璐ㄧ矑
Flag-SIRT1
Catalog No.PVT11538
Packing 2ug
Flag-SIRT1 Alternative name
Flag-SIRT1Flag-SIRT1 vector, Flag-SIRT1 plasmid, Flag-SIRT1 map, Flag-SIRT1 sequence,
Promoter -
Replicon -
Terminator -
Resistance Amp
Strain DH5alpha
Culture conditions LB,37degrees centigrade
Forward primer -
Reverse primer -
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文献和实验Methods to Investigate the Role of SIRT1 in Endothelial Senescence
Sirtuins are a family of proteins with NAD+ -dependent deacetylase or mono-ADP-ribosyltransferase activity. SIRT1, the mammalian ortholog most closely related to Sir2 (the first gene of this family discovered in yeast), exhibits anti
SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome
, in combination with affinity enrichment of acetyllysine peptides and mass spectrometry, to study the dynamic changes of the Lysine acetylome in response to Sirt1. The method should be applicable to quantify changes to other post translational modifications
Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
a methodological workflow based on an SF?TAP tag comprising a doublet Strep?tag II and a FLAG moiety optimized for rapid as well as efficient tandem affinity purification of native proteins and protein complexes in higher eukaryotic cells. Depending
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