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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pSV40-Gpr19-m-A58G
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pSV40-Gpr19-m-A58G/ pSV40-Gpr19-m-A58G璐ㄧ矑
pSV40-Gpr19-m-A58G
Catalog No.PVT11483
Packing 2ug
pSV40-Gpr19-m-A58G Alternative name
pSV40-Gpr19-m-A58GpSV40-Gpr19-m-A58G vector, pSV40-Gpr19-m-A58G plasmid, pSV40-Gpr19-m-A58G map, pSV40-Gpr19-m-A58G sequence,
Promoter -
Replicon -
Terminator -
Resistance Amp
Strain DH5alpha
Culture conditions LB,37degrees centigrade
Forward primer -
Reverse primer -
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文献和实验PLASMID MIDI-PREP FROM BACTERIA
1.0 mL of 1M Tris pH 7.5, 200 m L of 0.5M EDTA pH 8.0, and 99 mL of dH2 O 5. 4M LiCl (100 mL): Mix 17 g of LiCl with 100 mL of dH2 O (Autoclave ) 6. TB media (1 L): Mix 12 g of Bacto Tryptone, 24 g
PREPARATION OF PLASMID DNA FOR SEQUENCING
(LB; Luria-Bertani) 10 g tryptone 5 g yeast extract 5 g NaCl 1 L water Autoclave RNAse (10 mg/ml) Dissolve RNAse A in water. Boil for 5 minutes. Store at -20℃. Some protocols also add an equal volume of 40% glycerol and a half
EDTA up to 100 ml with Q store at room temperature 3 M NaOAc 5.2 24.6 g anhydrous sodium acetate pH to 5.2 with acetic acid and bring up to 100 ml with Q Procedure • Inoculate a 2 ml overnight
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