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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pCY 3010-07
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pCY 3010-07/ pCY 3010-07璐ㄧ矑
pCY 3010-07
Catalog No. PVT10557
Packing 2ug
Function /
Resistance /
Screen /
Strain /
Culture temperature
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
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文献和实验How to insert one single nucleotide into a plasmid-Mole
I did stupid thing, after cloning a sequence into a plasmid, just find out not in frame with the following sequence. just need insert one single nucleoacid will be ok. Any method to insert only one nucleoacid? or I need do cloning from the start
280 比值介于1.75~1.85 之间, 超螺旋质粒DNA 的比例大于80%, 该工艺纯化的质粒DNA 能达到或接近FDA 规定的人用质粒DNA 的各项指标, 整个过程不使用动物源性酶和苯酚、氯 仿、无水乙醇等有毒或易燃、易爆试剂, 成本低廉, 工艺环保。 关键词: 十六烷基三甲基溴化铵, 质粒DNA, 纯化, 质量检测 Purification of Large-scale Plasmid DNAs by Selective Precipitation
Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
and volumes for ppt.need 1.4ml ppt/100mm (half is 2x HeBS, half is DNA/CaCl2 mixture).20-30ug of total DNA required per 100mm plate.5-20ug reporter plasmid.3-4ug alpha-globin internal control.pUC19 to bring to 20-30ug total DNA.Aliquot volume of 2x HeBS
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