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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pT7-His-MMp9
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pT7-His-MMp9/ pT7-His-MMp9璐ㄧ矑
pT7-His-MMp9
Catalog No. PVT10153
Packing 2ug
Function ORF expression plasmid
Resistance /
Screen /
Strain /
Culture temperature
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
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文献和实验Reaction Mix: It is again convenient to make up a master mix to be aliquotted out to amplify all samples, if they are to use the same primers. If not, modify master mix by simply leaving out primers. Total volume: 50ul / rxn x N + 5-10
Cardiac Stem Cell Niche, MMP9, and Culture and Differentiation of Embryonic Stem Cells
matrix (ECM) and surrounding niche/microenvironment play pivotal roles in ESC differentiation. Matrix metalloproteinase-9 (MMP9) induces fibrosis that causes stiffness of the ECM and impairs differentiation of cardiac stem cells into cardiomyocytes
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
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