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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pEGFP-CL10
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pEGFP-CL10/ pEGFP-CL10璐ㄧ矑
pEGFP-CL10
Search namepEGFP-CL10,Plasmid pEGFP-CL10,pEGFP-CL10 vector
Bacterial Resistance:KanamycinGrowth Strain:
Species human(H. sapiens)
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文献和实验, and resuspend in 10 µl of TE, pH 8.0. Use 2 µl of each for analysis on a 1% agarose gel. Purification of cosmids and low-copy-number plasmids From: QIAGEN Plasmid Mini Handbook Cosmids and low-copy-number plasmids often require large culture volumes
at 37 degrees for ~20 minutes. Reagents/Solutions Solution 1 50mM glucose 25mM Tris*Cl (pH 8.0) 10mM EDTA (pH 8.0) Autoclave small batches of 100mL and store at 4 C. Solution 2 0.1M NaOH
in 0.4 ml of TE buffer (10 mM Tris-Cl, 1 mM EDTA, pH 7.5). Add 10ul of RNAse A solution (20 mg/ml stock stored at -20 ℃), vortex and incubate at 37 ℃ for 20 to 30 minutes to digest remaining RNA. 8. Extract proteins from the plasmid DNA using PCIA
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