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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
PFU enzyme
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
PFU enzyme/ PFU enzyme璐ㄧ矑
PFU enzyme
Search namePFU enzyme,Plasmid PFU enzyme,PFU enzyme vector
Bacterial Resistance:Growth Strain:
Use:Protease expression plasmid
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文献和实验between the bands of these different forms is influenced by percentage of agarose in the gel time of electrophoresis degree of supercoiling and the size of the DNA .One cannot accurately determine size from uncut plasmid DNA .When cut with an enzyme
Enzyme Kinetics assay of the WT
To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media Inc., Lavellette, NJ), frozen on dry ice and stored at -80 C. Cell pellets were thawed
Side directed mutagenesis with 17kb plasmid-Molecular Bi
the following cycling parameters: 1. 95℃ 1 min 2. 95℃ 50 sec 3. 60℃ 50 sec 4. 68℃ 1min/kb plasmid (so for my plasmid 17min) 5. 68℃ 7min As I have already said no colonies! Then I read that the Pfu-polymerase needs 2min/kb of the plasmid in the elongation
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