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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
shp53 pLKO.1 puro
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
shp53 pLKO.1 puro
Product Name: shp53 pLKO.1 puro
Cat No.: PVT18229
Alternative Name: shp53 pLKO.1 puro,shp53 pLKO.1 puro vector,shp53 pLKO.1 puro plasmid,shp53 pLKO.1 puro map,shp53 pLKO.1 puro sequence
Plasmid form: Freeze-dried powder
Storage Condition: -20 Centigrade
Bulk order
Promoter: U6
Bacterial Resistance(s): Amp
Screening: Puro
Growth Strain(s): Stbl3
Culture Medium: LB
Temperature: 37 Centigrade
Vector Size:
*Product is for research use only!
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文献和实验p53 Plasmid Preparation and Techniques for Analysis of Gene Transfer and Expression
Hepatocellular carcinoma (HCC) is one of the most common causes of cancer death worldwide and is especially prevalent in certain areas of Africa and Asia. The most important etiological factor is infection with the hepatitis B or C virus
Transactivation and Transrepression Studies with p53
can be cloned upstream of a luciferase gene in a plasmid. The most common reason for transfection experiments is to study gene expression patterns in the presence or absence of a particular gene product (e.g., p53). Three methods of transfection are outlined
:pCas9/gRNA1 载体本身不带筛选标签。我们用带有嘌呤霉素和 EGFP 标签的载体 pLV-EGFP(2A)puro 和 pCas9/gRNA1 载体共转染,再用嘌呤霉素筛选转染成功的细胞。 简要步骤 设计靶点→构建 pCas9/gRNA1 载体→pTYNE 验证靶点→pCas9/gRNA1 载体转染 293T 细胞→挑选培养细胞克隆→测序验证基因敲除 靶点设计和基因敲除载体构建 人 TP53 基因有多个 mRNA 和蛋白拷贝,2 个转录起始位点,以及多个
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