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- LSM BIO
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- PVT17582
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pHEE401E
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pHEE401E
Product Name: pHEE401E
Cat No.: PVT17582
Alternative Name: pHEE401E,pHEE401E vector,pHEE401E plasmid,pHEE401E map,pHEE401E sequence
Plasmid form: Freeze-dried powder
Storage Condition: -20 Centigrade
Bulk order
Promoter: inquiry
Bacterial Resistance(s): Kan
Screening: Hyg
Growth Strain(s): Stbl3
Culture Medium: LB
Temperature: 37 Centigrade
Vector Size:
*Product is for research use only!
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文献和实验Maxiprep of plasmid DNA from E. coli
Grow a single colony of E. coli overnight in 50mL LB broth + selective markers at 37°C. The next morning, put 850μL of the culture in each of two Eppendorf tubes, add 150μL sterile 50% glycerol, and store at -80°C. Pour as much culture as will fit
Designing Expression Plasmid Vectors in E. coli
, difficulties are encountered when theoretical strategies are put into practice, so gene expression is still quite empirical. E. coli remains an important host system for the industrial production of proteins from cloned genes, and considerable lore
Transformation DNA fragments (or plasmid DNA) into competent E. coli
Transformation DNA fragments (or plasmid DNA) into competent E. coli * Caution: Use aerosol protecting tips if selection of transformed cells is not based on X-gal strategy. 1.Remove competent cells (E.coli DH5aTM from GIBCO BRL) from -70 ℃
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