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- LSM BIO
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- PVT17575
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
Sema3e(L)-Fc-His
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
Sema3e(L)-Fc-His
Product Name: Sema3e(L)-Fc-His
Cat No.: PVT17575
Alternative Name: Sema3e(L)-Fc-His,Sema3e(L)-Fc-His vector,Sema3e(L)-Fc-His plasmid,Sema3e(L)-Fc-His map,Sema3e(L)-Fc-His sequence
Plasmid form: Freeze-dried powder
Storage Condition: -20 Centigrade
Bulk order
Promoter: inquiry
Bacterial Resistance(s): Amp
Screening: inquiry
Growth Strain(s): DH5alpha
Culture Medium: LB
Temperature: 37 Centigrade
Vector Size:
*Product is for research use only!
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文献和实验add 1 ul of plasmid DNA to 50 ul (w/ 10^8 CFU/ml) of ice-cold cell suspension in a .2 cm cuvette electroporate - 12.5 kV/cm, pulse number of 10, puse interval of 500 ms, plasmid DNA concentration of 25 ng/ul dilute cell
, and resuspend in 10 µl of TE, pH 8.0. Use 2 µl of each for analysis on a 1% agarose gel. Purification of cosmids and low-copy-number plasmids From: QIAGEN Plasmid Mini Handbook Cosmids and low-copy-number plasmids often require large culture volumes
Hemp (Cannabis sativa L.) suspension culture cells were transformed with Agrobacterium tumefaciens strain EHA101 carrying the binary plasmid pNOV3635. The plasmid contains a phosphomannose isomerase (PMI) selectable marker gene. Cells
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