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100 µL
Ephrin B2|Ephrin B2 antibody|Ephrin B2抗体|Anti-Human 兔多抗(产品说明)
抗体种属:Human
抗体靶点:EFNB2
抗体应用:IHC-P
抗原货号:
抗原描述:E. coli-derived Human Ephrin B2/EFNB2 fragment
抗体宿主:Rabbit
抗体Ig类型:Rabbit IgG
抗体纯化方法:Protein A & Antigen Affinity
抗体标记:
抗体制备:Produced in rabbits immunized with E. coli-derived Human Ephrin B2/EFNB2 fragment, and purified by antigen affinity chromatography.
抗体特异性:Human Ephrin B2/EFNB2
抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
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文献和实验Detection of Class Switching and IgE Synthesis in Human B Cells
events leading to IgE synthesis. This chapter will illustrate how human B cells (na�ve or memory) can be purified, stored, and cultivated in medium that is permissive for IgE synthesis and stimulated with IL-4 or IL-13 and CD40 activation
Isolating primary B cells from human blood
1. start with freshly drawn human blood not older than 8 hours, treated with an anti-coagulant (use 500ul of 2.7% EDTA for 10ml blood if you are not using EDTA coated tubes when collecting blood) 2. dilute cells with 4 volumes
-p-P38,用5%脱脂奶粉PBST溶液(图A)按照1:1000比例稀释;用Solution 1(图B)按照1:5000比例稀释。 二抗:用5%脱脂奶粉PBST溶液(图A)和Solution 2(图B)按照1:4000稀释。 样品:大鼠脑组织。 一抗:anti-SERT兔多抗,用2.5%脱脂奶粉PBST溶液(图A)按照1:1000比例稀释;用Solution 1(图B)按照1:10,000比例稀释。 二抗:anti-rabbit IgG,用2.5%脱脂奶粉PBST溶液(图
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