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100 µL
抗体种属:Human
抗体靶点:GSTM5
抗体应用:WB,ELISA,IHC-P,IP
抗原货号:15453-H07E
抗原描述:Recombinant Human GSTM5/GST class-mu 5 Protein (Catalog#15453-H07E)
抗体宿主:Rabbit
抗体Ig类型:Rabbit IgG
抗体纯化方法:Protein A & Antigen Affinity
抗体制备:Produced in rabbits immunized with purified, recombinant Human GSTM5/GST class-mu 5 (rh GSTM5/GST class-mu 5; Catalog#15453-H07E; AAH58881.1; Met1-Lys218). GSTM5/GST class-mu 5 specific IgG was purified by Human GSTM5/GST class-mu 5 affinity chromatography.
抗体特异性:Human GSTM5/GST class-mu 5
抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验μl of ice-cold 1 x PBS ,remove 10 μl of these resuspend cells into labeled tubes (for later use in SDS-PAGE analysis). -Note: Except where noted,keep all samples and tubes on ice. Lyse the Cell s using the sonicator equipped
,轻柔振荡60min (10)3000rpm,离心3min,回收上清 (11)1ml 细胞裂解缓冲液洗涤3次 (12)弃尽上清,加入25μl 2×SDS上样缓冲液 (13)煮沸3min,12000rpm,离心3min (14)SDS-PAGE,上样顺序:细胞裂解液input、x、GST、x、GST-融合蛋白(x:LSB) (15)考马斯亮蓝染色或免疫印迹 [附录] GST裂解缓冲液(前四个组
4 °C离心30 min,以分离上清和沉淀。1.5 ml 裂解缓冲液重悬沉淀。分别取10 μl上清和重悬液为样品。 7) 加入10μl谷胱甘肽琼脂糖4B 珠,4 °C孵育 1 hr并用 200 μl 1 x PBS洗3次。离心后收集上清。 8) 10 μl洗脱缓冲液(20 mM谷胱甘肽, 75 mM Tris pH 8.0, 150 mM NaCl)清洗柱子。 9) 每一步取的样品进行合适浓度的 SDS-PAGE,以确定表达纯化的条件和蛋白
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