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- Sino Biological
- 15179-RP01
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
100 µL
抗体种属：Human
抗体靶点：ACTR3
抗体应用：ELISA
抗原货号：15179-H20B
抗原描述：Recombinant Human ACTR3 / Actin-related protein 3 Protein (Catalog#15179-H20B)
抗体宿主：Rabbit
抗体Ig类型：Rabbit IgG
抗体纯化方法：Protein A
抗体制备：Produced in rabbits immunized with purified, recombinant Human ACTR3 / Actin-related protein 3 (rh ACTR3 / Actin-related protein 3; Catalog#15179-H20B; NP_005712.1; Ala2-Ser418). Total IgG was purified by Protein A affinity chromatography.
抗体特异性：Human ACTR3 / Actin-related protein 3
抗体保存：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验Protein purification; actin Overview ACTIN The most abundant muscle and non-muscle cytoskeletal protein. MW 42 kDa, 374/375 amino acids; various isoforms; structure resolved at 2.8 Angstrom. [see also protocol ID 207,208,209]
Combine actin + GST-fusion protein and incubate for 1 hr. x 4¡C. Add 20ul 50% glutathione agarose equilibrated into binding buffer. Incubate 15 min x 4¡C with frequent mixing. Wash beads 4 times with binding buffer, spin down 4-8000 rpm
by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
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