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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pBSFI-myr-akt1
- 库存：
100
- 供应商：
LSMBIO
- 规格：
2ug
pBSFI-myr-akt1
Catalog No. PVT10429
Packing 2ug
pBSFI-myr-akt1 Information
Function DNA Cloning plasmid
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
pBSFI-myr-akt1 Sequence
LOCUS Exported 4477 bp ds-DNA circular SYN 30-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4477)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..4477
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 63..95
/label=myristolation signal
misc_feature 96..1535
/label=Akt1
CDS 1536..1562
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
promoter complement(1632..1650)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1660..1676)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(1817..2272)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2299..2403
/gene="bla"
/label=AmpR promoter
CDS 2404..3264
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3435..4023
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4409..4425
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 4446..4464
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 agctggccat tacggccgcg gccgcctcta gaggatctgg taccacgcgt atcgataagc
61 ttatggggag cagcaagagc aagcccaagt ctagaatgaa cgacgtagcc attgtgaagg
121 agggctggct gcacaaacga ggggaatata ttaaaacctg gcggccacgc tacttcctcc
181 tcaagaacga tggcaccttt attggctaca aggaacggcc tcaggatgtg gatcagcgag
241 agtccccact caacaacttc tcagtggcac aatgccagct gatgaagaca gagcggccaa
301 ggcccaacac ctttatcatc cgctgcctgc agtggaccac agtcattgag cgcaccttcc
361 atgtggaaac gcctgaggag cgggaagaat gggccaccgc cattcagact gtggccgatg
421 gactcaagag gcaggaagaa gagacgatgg acttccgatc aggctcaccc agtgacaact
481 caggggctga agagatggag gtgtccctgg ccaagcccaa gcaccgtgtg accatgaacg
541 agtttgagta cctgaaacta ctgggcaagg gcacctttgg gaaagtgatt ctggtgaaag
601 agaaggccac aggccgctac tatgccatga agatcctcaa gaaggaggtc atcgtcgcca
661 aggatgaggt tgcccacacg cttactgaga accgtgtcct gcagaactct aggcatccct
721 tccttacggc cctcaagtac tcattccaga cccacgaccg cctctgcttt gtcatggagt
781 atgccaacgg gggcgagctc ttcttccacc tgtctcgaga gcgcgtgttc tccgaggacc
841 gggcccgctt ctatggtgcg gagattgtgt ctgccctgga ctacttgcac tccgagaaga
901 acgtggtgta ccgggacctg aagctggaga acctcatgct ggacaaggac gggcacatca
961 agataacgga cttcgggctg tgcaaggagg ggatcaagga tggtgccact atgaagacat
1021 tctgcggaac gccggagtac ctggcccctg aggtgctgga ggacaacgac tacggccgtg
1081 cagtggactg gtgggggctg ggcgtggtca tgtatgagat gatgtgtggc cgcctgccct
1141 tctacaacca ggaccacgag aagctgttcg agctgatcct catggaggag atccgcttcc
1201 cgcgcacact cggccctgag gccaagtccc tgctctccgg gctgctcaag aaggacccta
1261 cacagaggct cggtgggggc tctgaggatg ccaaggagat catgcagcac cggttctttg
1321 ccaacatcgt gtggcaggat gtgtatgaga agaagctgag cccacctttc aagccccagg
1381 tcacctctga gactgacacc aggtatttcg atgaggagtt cacagctcag atgatcacca
1441 tcacgccgcc tgatcaagat gacagcatgg agtgtgtgga cagtgagcgg aggccgcact
1501 tcccccagtt ctcctactca gccagtggca cagcctaccc atacgatgtt ccagattacg
1561 cttctagatg aattcacgcg taagcttatc gatgtcgacg ggcccggccg cctcggccgt
1621 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
1681 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
1741 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
1801 cctgaatggc gaatggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
1861 tgttaaatca gctcattttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
1921 aaaagataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
1981 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
2041 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
2101 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
2161 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
2221 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
2281 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
2341 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
2401 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
2461 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
2521 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
2581 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
2641 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
2701 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
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文献和实验该产品被引用文献
Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation
Author：YangZhang1YiXu1WenyunLu23Jonathan M.Ghergurovich24LiliGuo56Ian A.Blair5Joshua D.Rabinowitz23XiaoluYang17
Received 22 January 2020, Revised 4 August 2020, Accepted 30 September 2020, Available online 6 November 2020.
Published: November 6, 2020
Cell Metabolism
Available online 6 November 2020 In Press
doi: doi.org/10.1016/j.cmet.2020.10.002
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pBSFI-myr-akt1
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