pRK5-HA-Ubiquitin-K48
Catalog No. PVT10388
Packing 2ug
pRK5-HA-Ubiquitin-K48 Information
Function ORF expression plasmid
Resistance /
Screen /
Strain /
Culture temperature
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
pRK5-HA-Ubiquitin-K48 Sequence
LOCUS Exported 4996 bp ds-DNA circular SYN 27-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4996)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 27, 2017
FEATURES Location/Qualifiers
source 1..4996
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 14..393
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 394..597
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 828..846
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
regulatory 963..972
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 975..1001
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 1032..1259
/codon_start=1
/product="human ubiquitin variant containing Lys-48, with
all other lysines mutated to arginine"
/label=ubiquitin-K48
/translation="MQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIF
AGKQLEDGRTLSDYNIQRESTLHLVLRLRGG"
regulatory 1296..1305
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
polyA_signal 1309..1443
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 1512..1869
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1720..1855
/label=SV40 ori
/note="SV40 origin of replication"
rep_origin 2118..2573
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2855..2959
/gene="bla"
/label=AmpR promoter
CDS 2960..3820
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3991..4579
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
901 acctcggttc tatcgattga attcccgaac cgacagtcgg tctcttcacc aaggccattc
961 gcgccaccat gggctacccc tatgatgtgc ctgactacgc agatctcaat ggtggtggtg
1021 gtgggtcgac catgcagatc ttcgtcagaa cgttaaccgg tagaaccata actctagaag
1081 ttgaaccatc cgataccatc gaaaacgtta gagctagaat tcaagacaga gaaggcattc
1141 cacctgatca acaaagattg atctttgccg gtaagcagct cgaggacggt agaacgctgt
1201 ctgattacaa cattcagaga gagtccaccc tgcacctggt cctccgtctc agaggtggtt
1261 gagcggccgc taagtaagta aggatcccca gcttggccgc catggcccaa cttgtttatt
1321 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
1381 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
1441 atcgggaatt aattcggcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg
1501 ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
1561 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
1621 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
文献和实验
技术资料