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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pBV220-EK
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pBV220-EK
Catalog No. PVT10508
Packing 2ug
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pBV220-EK Information
Function Protease plasmids
Promoter: pR/pL
Replicon: ColE ori
Terminator: rrnB T1, rrnB T2
Plasmids: protease expression plasmids
Plasmid label: EK
Prokaryotic resistance: ampicillin Ampicillin
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expression host: Escherichia coli
Culture conditions: 37 centigrade, aerobic, LB
Induction mode: 42 C induction
5'sequencing primers: pBV220-F: AAGAAGGGCAGCATTCAAAG
3'sequencing primers: pBV220-R: CTGCGTTCTGATTTAATCTG
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文献和实验温度诱导型原核表达载体pBV220工作原理及全序列 实验原理 PLPR启动子是大肠杆菌l噬菌体中控制早期转录的启动子,具有极强的起始RNA转录的功能,基因工程中常用它来构建高效表达载体,它受抑制物CI蛋白的负调控。在实际中CI蛋白被改造成温度敏感型(CIts),由DNA片段CIts857(857bp长)编码。CIts在30℃时具有抑制启动子的活性,在42℃时失活而失去抑制启动子的活性,因此,改变工程菌的培养温度即可控制目的基因的表达,这一点比用诱导剂诱导表达的系统要节省操作步骤和成本
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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