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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pCR4-PKD2
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pCR4-PKD2
Catalog No. PVT10110
Packing 2ug
Function DNA Cloning plasmid
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon pUC
Copy high
Promoter -
Induction -
Forward primer M13F
Reverse primer M13R?
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文献和实验Random Mutagenesis by Whole-Plasmid PCR Amplification
Mutagenesis is a popular tool used in the analysis of protein structure and function. Polymerase chain reaction (PCR)-based mutagenesis can be used to introduce mutations with the use of the appropriate primer. Although the majority
μL 体系 2× MasterMix 25 μL 上游 Primer ( 10 μM 1-5 μL 下游 Primer ( 10 μM ) 1-5 μL 模板 lamid DNA ~ 50 ng genomic DNA ~ 1000 ng cDNA ~ 500 ng 粗提液 ~ 2 μL 灭菌蒸馏水至 50 μL PCR 扩增实例: 一、plasmid DNA 扩增 1.提取的质粒 DNA 扩增 以pUC19 质粒为模板扩增 100,250,500
Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP)
The conventional method for cloning a DNA fragment is to insert it into a vector and ligate it (1 ). Although this method is commonly used, it is labor intensive because the ratio and concentrations of the DNA insert and the vector
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