- ￥800
- LSM BIO
- 进口
- PVT10068
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pENTR223-TRNAU1AP (331-864)
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pENTR223-TRNAU1AP(331-864)
Catalog No. PVT10068
Packing 2ug
Function DNA Cloning plasmid
Resistance Spe
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer ?
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文献和实验Frozen competent E. coli cells 【Carnegie Institution of Washington】
DNA.Leave 30’ on ice. Heat shock,45 sec at42℃ then chill cells on ice about 2’. Spin down (15 sec,eppendorf fuge)and remove SN.(Removing the Manganese seems to boost efficiency about 10X)and resuspend cells in 200μl LB. For a supercoiled plasmid
FROZEN COMPETENT E. COLI CELLS
. (Removing the Manganese seems to boost efficiency about 10X) and resuspend cells in 200 ul LB. For a supercoiled plasmid, plate 1 ul of cells. For a ligation, plate 20 ul and the rest. TB (transformation buffer: filter
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
-expressing constructs into early embryos permits key developmental events such as gastrulation to be dynamically imaged using time-lapse video-microscopy. This protocol describes the ex ovo electroporation of a DNA plasmid into avian embryos as young
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