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- LSM BIO
- 进口
- PVT10196
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pCXWB-EBNA1
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pCXWB-EBNA1
Catalog No. PVT10196
Packing 2ug
?
pCXWB-EBNA1 Information
Function ORF expression plasmid
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
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文献和实验Filter Binding Assay for EBNA-1
, 3ml syringePre-incubate filter with 100ul Binding Buffer at filter binding temperature (4C) until ready to bind DNA/EBNA-1 solution (e.g. conduct in 7th floor cold room).Incubate DNA fragment of interest with DNA with no known binding sites in a total
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
Procedure I. INTRODUCTION The 293 EBNA cell line is established from primary embryonal human kidney cells transformed with sheared human adenovirus type 5 DNA and Epstein-Barr virus nuclear antigen 1, allowing episomal amplification
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
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