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- PVTB01069-2a
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pcDNA3.0-IQGAP1(746aa-1657aa)-HA
- 库存：
100
- 供应商：
LSMBIO
- 规格：
2ug
pcDNA3.0-IQGAP1(746aa-1657aa)-HA Plasmid
Catalog No. PVTB01069-2a
Vector Backbone pcDNA3
Backbone manufacturer Invitrogen
Vector type Mammalian Expression
Backbone size w/o insert 5400bp
Cloning site 5 Not I
Site destroyed during cloning NO
Cloning site 3 Xho I
Bacterial resistance Ampicillin
Growth strain Top10
Growth temperature 37 Centigrade
High or low copy High Copy
Selectable markers Neomycin
Background Mammalian Expression vector of Homo sapiens IQ motif containing GTPase activating protein 1 (746aa-1657aa) with C terminal HA tag
Gene Information
Alternative Gene Name HUMORFA01;p195;SAR1
Insert Gene name IQGAP1(746aa-1657aa)
Insert size 2739bp
Accessions BC139731
Tag HA(C-term)
Species Homo sapiens (human)
Gene ID 8826
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文献和实验AA Metabolite Quantitation of Media Post-AA Labeling
TLC plate for TLC system #1, use 20µl to spot a linear K TLC plate for TLC system #2. TLC : A) Allow TLC tanks to equilibrate 1-2 hrs with solvent prior to running. SYSTEM #1 --> SeparatesPA, TXB2, HHT, HETE & AA. ethylacetate
AA Metabolite Quantitation of Cell Pellets Post-AA Labeling
. SYSTEM #1 --> SeparatesPA, TXB2, HHT, HETE & AA. ethylacetate/2,2,4-trimethylpentane/acetic acid/ H2O at a ratio of 90:50:20:100 SYSTEM #2 --> Separates DAG & AA. benzene/diethyl ether/ethanol/NH3 at a ratio of 100:80:4:0.2 B) Following TLC remove plates
AA & Metabolite Quantitation of Media Post-AA
AA & Metabolite Quantitation of Media Post-AA Labeling Contributor: Suprya Jayadev Date: November 4, 1994 1) Remove 2 500 µl aliquots of supernatant into scintillation vials, add scintillation fluid and count
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