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- PVTB00460-2r
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pCMV-HA-IMPDH2(C140S)
- 库存：
100
- 供应商：
LSMBIO
- 规格：
2ug
pCMV-HA-IMPDH2(C140S) Plasmid
Catalog No. PVTB00460-2r
Vector Backbone pCMV-HA
Backbone manufacturer Clontech
Vector type Mammalian Expression
Backbone size w/o insert 3800bp
Cloning site 5 Sal I
Site destroyed during cloning NO
Cloning site 3 Kpn I
Bacterial resistance Ampicillin
Growth strain Top10
Growth temperature 37 Centigrade
High or low copy High Copy
Selectable markers None
Background Mammalian Expression vector of Homo sapiens inosine monophosphate dehydrogenase 2(one point mutation C140S) with N terminal HA tag
Gene Information
Alternative Gene Name IMPD2; IMPDH-II
Insert Gene name IMPDH2(C140S)
Insert size 1545bp
Accessions BC006124
Tag HA(N-term)
Species Homo sapiens (human)
Gene ID 3615
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文献和实验病毒质粒 pCMVdR8.2 病毒质粒 Amp 包装质粒，可和CMV/R-HA和Pcdna3.1等配套使用 pHR-Luc 病毒质粒 Amp 包装质粒，可和CMV/R-HA和Pcdna3.1等配套使用 pCMV
Preparation of plasmid DNA （质粒制备）
4 TO 900 ml of base broth (base broth = 12g bacto-tryptone, 24g bacto-yeast extract, 4.0 ml glycerol, q.s. to 900 ml with deionized water and then autoclave). Note: The above volume of glycerol is most easily measured out by weighing : 4.0ml glycerol
Preparation of plasmid DNA（质粒制备）
broth = 12g bacto-tryptone, 24g bacto-yeast extract, 4.0 ml glycerol, q.s. to 900 ml with deionized water and then autoclave). Note: The above volume of glycerol is most easily measured out by weighing : 4.0ml glycerol = 5.0 g. Methods
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