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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
lentiCRISPR V1.0
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
lentiCRISPR V1.0
Search name
lentiCRISPR V1.0,Plasmid lentiCRISPR V1.0,lentiCRISPR V1.0 vector
Bacterial Resistance:Ampicillin
Growth Strain:
Use:CRISPR-CAS23-sgRNA plasmid
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文献和实验++ ) and without (PBS– ) Ca2+ and Mg2+ (Invitrogen) DNA plasmid constructs (pC5V, pC5A, pC17V, pC17A, pC32V, pC32A, and pV1) Plasmid DNA
with CXCR4‐hRluc alone, pCXCR4‐hRluc/pGBR2‐EYFP, or pCXCR4‐EYFP/pGBR2‐hRluc served as negative controls, while transfection with pEYFP‐hRluc fusion plasmid was used as a positive control for BRET1 . After the addition of coelenterazine‐ h , the BRET1 signal
cDNA Expression Cloning in Mammalian Cells
plates, or 10‐cm tissue culture dishes, sterile Additional reagents and equipment for bioassay of transfected cells (see Chapter 7), isolation and purification of plasmid DNA, counting
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