pK18mobSacB Plasmid

pK18mobSacB

Search name

pK18mobSacB,Plasmid pK18mobSacB,pK18mobSacB vector

pK18mobSacB Information

Carrier name: pK18mobSacB

Plasmid type: Bacillus subtilis gene knockout carrier

Cloning methods: polyclonal sites, restrictive endonucleases

Carrier size: 5.6kb

5'sequencing primers and sequences: --

3'sequencing primers and sequences: --

Carrier label: -

Carrier resistance: Kanamycin

Use:Homologous recombination HR plasmid

pK18mobSacB Description

The Cloning vector allowing mobilization into a wide range of Gramand Gram+ bacteria. After mobilization, the plasmid can be maintained by integration into the host chromosome viahomologous recombination. Excision of the intervening plasmid sequence by a double crossoverevent can be facilitated by selection on medium containing 10% sucrose.The sacB gene has been modified to eliminate the HindIII and EcoRI sites in the coding region.Designation: pK18mobsacB

pK18mobSacB Sequence

  LOCUS       pK18mobsacB   5721 bp   DNA                       17-AUG-2006  DEFINITION  Cloning vector pK18mobsacB.  ACCESSION  VERSION  KEYWORDS    .  SOURCE      Cloning vector pK18mobsacB.    ORGANISM  Cloning vector pK18mobsacB              Unclassified.  REFERENCE   1  (bases 1 to 5721)    AUTHORS   Schafer,A., Tauch,A., Jager,W., Kalinowski,K., Thierbach,G. and              Puhler,A.    TITLE     Small mobilizable multi-purpose cloning vectors derived from the              Escherichia coli plasmids pK18 and pK19: selection of defined              deletions in the chromosome of Corynebacterium glutamicum    JOURNAL   Gene 145, 69-73 (1994)  REFERENCE   2  (bases 1 to 5721)    AUTHORS   Ishikawa,J.    TITLE     Unofficial nucleotide sequence of pK18mobsacB    JOURNAL   Unpublished  REFERENCE   3  (bases 1 to 5721)    AUTHORS   Ishikawa,J.    TITLE     Direct Submission    JOURNAL   Submitted (17-AUG-2006) Bioactive Molecules, NIID  FEATURES             Location/Qualifiers       source          1..5721                       /organism="Cloning vector pK18mobsacB"                       /mol_type="Other DNA"       CDS             complement(185..469)                       /gene="lacZ"                       /codon_start=1                       /product="beta-galactosidase"       oriT            complement(2209..2736)                       /note="RP4 origin of transfer"       CDS             complement(2886..4307)                       /gene="sacB"                       /codon_start=1                       /product="levansucrase"       CDS             complement(4799..5593)                       /gene="neo"                       /note="confers resistances to neomycin and kanamycin"                       /codon_start=1                       /product="aminoglycoside 3'-O-phosphotransferase"  BASE COUNT     1343 a   1426 c   1414 g   1538 t  ORIGIN          1 gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc         61 cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt        121 agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac gtgttccgct        181 tcctttagca gcccttgcgc cctgagtgct tgcggcagcg tgaagctagc ttatcgcgcc        241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat        301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt        361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg        421 actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct        481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt        541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc        601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg        661 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg        721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca        781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac        841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac        901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg        961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac       1021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat       1081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag       1141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac       1201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt       1261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt       1321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc       1381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga       1441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac       1501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc       1561 cttttggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccc       1621 tgatagaaac agaagccact ggagcacctc aaaaacacca tcatacacta aatcagtaag       1681 ttggcagcat cacccgacgc actttgcgcc gaataaatac ctgtgacgga agatcacttc       1741 gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg       1801 cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga       1861 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctgatag aaacagaagc       1921 cactggagca cctcaaaaac accatcatac actaaatcag taagttggca gcatcacccg       1981 acgcactttg cgccgaataa atacctgtga cggaagatca cttcgcagaa taaataaatc       2041 ctggtgtccc tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg       2101 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat       2161 tttttgagtt atcgagattt tcaggagctc tttggcatcg tctctcgcct gtcccctcag       2221 ttcagtaatt tcctgcattt gcctgtttcc agtcggtaga tattccacaa aacagcaggg       2281 aagcagcgct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta       2341 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct       2401 tggtgtatcc aacggcgtca gcggggcagg ataggtgaag taggcccacc cgcgagcggg       2461 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg       2521 cgaggctggc cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc       2581 aagccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga       2641 gcgattgagg aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc       2701 ggccagggct acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gggcgtcccg       2761 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga       2821 tggcaggttg ggcgtcgctt ggtcggtcat ttcgctcggt accatcggca ttttcttttg       2881 cgtttttatt tgttaactgt taattgtcct tgttcaagga tgctgtcttt gacaacagat       2941 gttttcttgc ctttgatgtt cagcaggaag ctcggcgcaa acgttgattg tttgtctgcg       3001 tagaatcctc tgtttgtcat atagcttgta atcacgacat tgtttccttt cgcttgaggt       3061 acagcgaagt gtgagtaagt aaaggttaca tcgttaggat caagatccat ttttaacaca       3121 aggccagttt tgttcagcgg cttgtatggg ccagttaaag aattagaaac ataaccaagc       3181 atgtaaatat cgttagacgt aatgccgtca atcgtcattt ttgatccgcg ggagtcagtg       3241 aacaggtacc atttgccgtt cattttaaag acgttcgcgc gttcaatttc atctgttact       3301 gtgttagatg caatcagcgg tttcatcact tttttcagtg tgtaatcatc gtttagctca       3361 atcataccga gagcgccgtt tgctaactca gccgtgcgtt ttttatcgct ttgcagaagt       3421 ttttgacttt cttgacggaa gaatgatgtg cttttgccat agtatgcttt gttaaataaa       3481 gattcttcgc cttggtagcc atcttcagtt ccagtgtttg cttcaaatac taagtatttg       3541 tggcctttat cttctacgta gtgaggatct ctcagcgtat ggttgtcgcc tgagctgtag       3601 ttgccttcat cgatgaactg ctgtacattt tgatacgttt ttccgtcacc gtcaaagatt       3661 gatttataat cctctacacc gttgatgttc aaagagctgt ctgatgctga tacgttaact       3721 tgtgcagttg tcagtgtttg tttgccgtaa tgtttaccgg agaaatcagt gtagaataaa       3781 cggatttttc cgtcagatgt aaatgtggct gaacctgacc attcttgtgt ttggtctttt       3841 aggatagaat catttgcatc gaatttgtcg ctgtctttaa agacgcggcc agcgtttttc       3901 cagctgtcaa tagaagtttc gccgactttt tgatagaaca tgtaaatcga tgtgtcatcc       3961 gcatttttag gatctccggc taatgcaaag acgatgtggt agccgtgata gtttgcgaca       4021 gtgccgtcag cgttttgtaa tggccagctg tcccaaacgt ccaggccttt tgcagaagag       4081 atatttttaa ttgtggacga atcaaattca gaaacttgat atttttcatt tttttgctgt       4141 tcagggattt gcagcatatc atggcgtgta atatgggaaa tgccgtatgt ttccttatat       4201 ggcttttggt tcgtttcttt cgcaaacgct tgagttgcgc ctcctgccag cagtgcggta       4261 gtaaaggtta atactgttgc ttgttttgca aactttttga tgttcatcgt tcatgtctcc       4321 ttttttatgt actgtgttag cggtctgctt cttccagccc tcctgtttga agatggcaag       4381 ttagttacgc acaataaaaa aagacctaaa atatgtaagg ggtgacgcca aagtatacac       4441 tttgcccttt acacatttta ggtcttgcct gctttatcag taacaaaccc gcgcgattta       4501 cttttcgacc tcattctatt agactctcgt ttggattgca actggtctat tttcctcttt       4561 tgtttgatag aaaatcataa aaggatttgc agactacggg cctaaagaac taaaaaatct       4621 atctgtttct tttcattctc tgtatttttt atagtttctg ttgcatgggc ataaagttgc       4681 ctttttaatc acaattcaga aaatatcata atatctcatt tcactaaata atagtgaacg       4741 gcaggtatat gtgatgggtt aaaaaggatc gatcctctag cgaaccccag agtcccgctc       4801 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac       4861 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg       4921 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc       4981 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga       5041 cgagatcctc gccgtcgggc atccgcgcct tgagcctggc gaacagttcg gctggcgcga       5101 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac       5161 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg       5221 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag       5281 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag       5341 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg       5401 ctgcctcgtc ttggagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg       5461 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg       5521 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat       5581 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc       5641 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct       5701 taccagaggg cgccccagct g  //