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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pREP4
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pREP4
Search name
pREP4,Plasmid pREP4,pREP4 vector
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pREP4 Plasmid?Information
Plasmid Label: Translac Inhibitor
Prokaryotic resistance: kanamycin
Clonal strain: DH5 alpha
Culture conditions: 37 C, aerobic, LB
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文献和实验Objective: Mini-Prep procedure is used to isolate small plasmid DNA from bacteria while limiting contaminating proteins and genomic DNA. The plasmid quality is acceptable for restriction analysis, sequencing, cloning, or other purposes
1. Spin down 1.5 ml of overnight culture in 2ml or 1.5ml tube for 1 minute on high. 2. Asprirate supernatant and resuspend cell pellet in 100 µl Solution I (25 mM Tris-HCl, pH 8.0, 10 mM EDTA). 3. Add 200 µl Solution II (0.2 N NaOH, 1.0% SDS
PLASMID MIDI-PREP FROM BACTERIA
of culture should produce ~0.5-1.0 mg of plasmid DNA from a high copy plasmid) 23. Optional: determine the amount that is barely visible on a THIN EtBr-agarose gel Note: EtBr has a visibility limit of about 100-200 ng
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