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- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pFastBac 1-signal-CD45(1-400aa)-His6
- 库存:
100
- 供应商:
LSMBIO
- 规格:
2ug
pFastBac 1-signal-CD45(1-400aa)-His6 Plasmid
Catalog No. PVTB00057-2a
Vector Backbone pFastBac?1
Backbone manufacturer Invitrogen
Vector type Insect Expression
Backbone size w/o insert 4775bp
Cloning site 5 EcoR I
Site destroyed during cloning NO
Cloning site 3 Hind III
Bacterial resistance Ampicillin
Growth strain DH5alpha
Growth temperature 37 Centigrade
High or low copy High Copy
Selectable markers Gentamicin
Background Insect Expression vector of Homo sapiens protein tyrosine phosphatase, receptor type C(1-400aa)
Gene Information
Alternative Gene Name PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Insert Gene name CD45
Insert size 1200bp
Accessions NM_002838.4
Tag His(C-term)
Species Homo sapiens (human)
Gene ID 5788
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文献和实验Measurement of Second Messengers in Signal Transduction: cAMP and Inositol Phosphates
here for measuring the conversion of [alpha?32 P]ATP into [32 P]cAMP after a two?step chromatographic separation. Also critical in signal tranduction is phosphoinositide turnover, which is linked to receptor activation resulting from changes in cytosolic calcium
16+ NK 细胞比例,并监测了细胞表面受体的差异表达,包括 CD8、CD11c、CD38、CD57 和 CD117,及其表达水平。样品制备:1. 加入 35 mL 新鲜人血(取自血库)和 20 mLPBS。2. 向 50 mL 锥形试管中加入 15 mL Ficoll-Paque,并转移 35 mL 经过 PBS 稀释的血样。3. 以室温 400 x G 持续离心试管 30 分钟。4. 用一个 (10-25 mL) 移液器或塑料的巴斯德吸管轻轻从试管中吸取间期细胞,并转移到一个新的 50 mL 锥形试管
. Atached to the membrane by a gpi-anchor Homologous to oher members of the eplg family. signal 1 22 ligand 6. carbohyd 36 36 carbohyd 161 161 carbohyd 175 175 G55A_Chick Neurite inhibitor gp55a (fragment) 274 aa. q98892
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