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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pSP64
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pSP64
Search name
pSP64,Plasmid pSP64,pSP64 vector
pSP64 Information
Carrier name: pSP64
Plasmid type: yeast two hybrid system carrier
High copy / low copy: high copy
Cloning methods: polyclonal sites, restrictive endonucleases
Carrier size: 2999bp
5'sequencing primers and sequences: Sp6 Fwd: ATTTAGGTGACACTATAG
Vector resistance: ampicillin (Ampicillian)
Stability: transient expression
Composition type: composition type
Virus / non virus: non virus
pSP64 Description
pSP64 Poly(A) Vector can be used as a standard cloning vector and for in vitro transcription from the SP6 promoter. The pSP64 Poly(A) Vector also can be used to generate poly(A)+ transcripts in vitro. The vector has a stretch of 30 dA:dT residues inserted between the SacI and EcoRI sites. Therefore, when foreign DNA is cloned into any polylinker site other than EcoRI (HindIII, PstI, SalI, AccI, HincII, XbaI, BamHI, AvaI, SmaI or SacI), linearization of the recombinant plasmid with EcoRI allows the use of SP6 RNA polymerase in vitro to prepare RNA copies of the inserted sequences that contain a synthetic 3鈥?"poly(A)" tail of 30 residues.
pSP64 Sequence
LOCUS pSP64 2999 bp DNA SYN DEFINITION pSP64 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..2999 /organism="pSP64" /mol_type="other DNA" promoter complement(63..81) /label="M13_reverse_primer" misc_feature complement(80..102) /label="M13_pUC_rev_primer" promoter complement(116..145) /label="lac_promoter" gene complement(1228..2088) /label="Ampicillin" /gene="Ampicillin" CDS complement(1228..2088) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(2130..2158) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature complement(2317..2339) /label="pGEX_3_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(2490..2741) /label="tet (887 - 636)" /gene="tet (887 - 636)" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 2673..2692 /label="pBRrevBam_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 2983..1 /label="Sp6_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 gaatacaagc ttgggctgca ggtcgactct agaggatccc cgggcgagct cgaattcgta 61 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 1021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 1081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 1141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 1201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 1261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 1321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 1381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 1441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 1501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 1561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 1621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 1681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 1741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 1801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 1861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 1921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 1981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 2041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 2101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 2161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 2221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 2281 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 2341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 2401 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 2461 gagagtgcac catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt 2521 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg 2581 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg 2641 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca 2701 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta 2761 gcgatgaccc tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga 2821 aactcagaag gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag 2881 ggtctgcttc agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg 2941 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactata //
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文献和实验CD64 分子 CD64 常用单克隆抗体或代号： MAb32.2,MAb22;(Fcg RI) 主要表达细胞： M [M] 分子质量(kDa)和结构： gp75(IgSF) 功 能： 吞噬、ADCC,Mac活化 CD64 Aka Fc gamma RI High affinity receptor binds to Fc region of IgG
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Indica Rice (Oryza sativa, BR29 and IR64)
Rice is the world’s most important food crop. Indica-type rice provides the staple food for more than half of the world population. To satisfy the growing demand of the ever-increasing population, more sustained production of indica-type rice
