pLV2-CMV-ST8SIA6-3×FLAG-Puro p

pLV2-CMV-ST8SIA6-3×FLAG-Puro plasmid

PVT104752

pLV2-CMV-ST8SIA6-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-ST8SIA6(human)-3×FLAG-Puro
Alias: NM_001004470.3;SIA8F; SIAT8-F; SIAT8F; ST8SIA-VI;ST8SiaVI
Gene lenght: 1194
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-ST8SIA6-3×FLAG-Puro plasmid Description

pLV2-CMV-ST8SIA6-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-ST8SIA6-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgcggcc ggggggcgca ctgctcgccc tgctcgccag 2881 cctgctgctg ctgctgctgc tgcgcctgct ctggtgcccg gcagacgcgc ccggccgcgc 2941 caggattctg gtggaggaaa gcagggaggc cacccacggc acccccgcag cgctgaggac 3001 gctccggagc ccggcgaccg cggtaccgcg cgccactaac agcacatatc tgaatgagaa 3061 gtcgctccaa ctgacggaga aatgcaaaaa tctgcaatat ggcattgagt ctttctctaa 3121 caaaacgaaa gggtattcag agaacgacta ccttcagatt atcacagata tacagagttg 3181 tccatggaaa cggcaagcag aagaatatgc aaattttaga gccaaacttg cttcctgctg 3241 tgatgctgtt caaaactttg ttgtttctca gaataacact ccagttggga ctaatatgag 3301 ttacgaggtg gaaagcaaaa aagaaatccc aattaagaag aacatttttc atatgtttcc 3361 agtgtcccag ccttttgtgg actaccctta taatcagtgt gcagtggtcg gaaatggggg 3421 aattctgaat aagtctctct gtggaactga aatagataaa tccgacttcg tttttaggtg 3481 taacctaccc ccaaccacag gagatgttag taaagatgtt ggcagtaaaa caaatcttgt 3541 gactataaat ccaagcatca taactctgaa atatgggaac ttaaaggaaa aaaaagccct 3601 atttctggag gacattgcaa cctatggaga tgcatttttt cttctgccag cattttcctt 3661 cagggccaac acgggtacct ctttcaaagt atactacacg ctcgaagagt ctaaagcaag 3721 acaaaaggtt ctatttttcc atcccaagta cctgaaagat ctggcccttt tctggagaac 3781 taaaggtgtg actgcatacc gcttgtccac cggcttgatg atcacaagtg ttgcagtgga 3841 actgtgtaaa aatgtgaagc tgtatggatt ctggcccttc tctaaaactg tagaagacat 3901 acctgtcagc catcactatt atgacaacaa gctacctaaa catggtttcc atcagatgcc 3961 caaagaatac agccagatcc tccaacttca catgaaagga atcctcaaac tgcaatttag 4021 caaatgtgaa gtcgccGGTG GAGGCggatc cGACTACAAA GACCATGACG GTGATTATAA 4081 AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGtag tctagataat tctaccgggt 4141 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac 4201 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca 4261 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa 4321 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct 4381 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg 4441 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg 4501 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg 4561 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc 4621 atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg 4681 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 4741 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 4801 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 4861 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 4921 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 4981 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 5041 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 5101 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 5161 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 5221 cgcacctggt gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct 5281 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 5341 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 5401 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 5461 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 5521 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 5581 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 5641 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 5701 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 5761 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 5821 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga 5881 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt 5941 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct 6001 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg 6061 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac 6121 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat 6181 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag 6241 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac 6301 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc 6361 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt 6421 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc 6481 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat 6541 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 6601 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 6661 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 6721 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg 6781 ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 6841 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 6901 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 6961 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 7021 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 7081 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 7141 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 7201 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 7261 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 7321 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 7381 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 7441 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 7501 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 7561 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 7621 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 7681 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 7741 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 7801 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 7861 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 7921 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 7981 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 8041 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 8101 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 8161 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 8221 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 8281 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 8341 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 8401 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 8461 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 8521 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 8581 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 8641 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 8701 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 8761 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 8821 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 8881 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 8941 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 9001 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 9061 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 9121 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc 9181 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc 9241 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta 9301 gtttttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.