pUC19 Plasmid

pUC19

Search name

pUC19,Plasmid pUC19,pUC19 vector

pUC19 Information

Promoter: Lac/lac promoter

Replicon: ColE1 ori

Plasmid classification: Escherichia coli vector; cloned plasmid

Plasmid size: 2686bp

Plasmid label: LacZ

Prokaryotic resistance: ampicillin Amp

Cloned strain: DH5 alpha

Culture conditions: 37 centigrade, aerobic, LB

5'sequencing primers: M13F:TGTAAAACGACGGCCAGT

3'sequencing primers: M13R:CAGGAAACAGCTATGACC

Note: selection of blue leukoplakia

pUC19 Description

The size of pUC18 and pUC19 is only 2686bp, the most commonly used plasmid vector with compact structure, almost free of excess DNA, and GenBank registration number of L08752 (pUC18) and X02514 (pUC19). From pBR322, lacZ (MSC) comes from M13mp18/19. The structure of these two plasmids is almost the same, but the direction of the polyclonal sites is opposite. These plasmids lack ROP gene that controls copy number, so the copy number is 500-700. The pUC series contains a partial coding sequence of the amino terminal of a lacZ protein, which can exhibit alpha complementarity in specific receptor cells. Therefore, after inserting foreign fragments into polyclonal sites, the recombinant plasmids can be screened through blue and white colonies formed by alpha complementarity. PUC18 and pUC19 vectors are suitable for cloning DNA fragments, sequencing DNA, and expressing foreign genes. Because of the polyclonal loci in the lacZ field, it can be easily screened by blue and white to judge whether there is a DNA fragment insertion in the carrier when the lacZ deficient cell is transformed into a host (such as JM109 and so on) and is cultured on a flat medium containing IPTG and X-Gal. At the same time, foreign genes can be expressed by lac promoter on the vector, and DNA fragments in insertion vectors can be sequenced. DNA sequencing can facilitate the use of M13 universal primers.

pUC19 Multiple site

pUC19 Sequence