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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
3个月
- 英文名：
EHA105 Agrobacterium Strain
- 库存：
50
- 供应商：
LSM BIO
- 规格：
100ul
EHA105 Agrobacterium Strain
STR3004 Packing 100ul
Storage condition -80鈩?/p>
EHA105 Agrobacterium Strain Information
Resistance: Rif
Culture medium: YEB
Strain category: Agrobacterium tumefaciens
Culture conditions: 28 centigrade aerobic, YEB
Transformation of plasmids: electric conversion and transformation
Preservation method: 30% glycerin, -80鈩?br>Basic application: transgene
Type Agrobacterium tumefaciens
EHA105 Agrobacterium Strain Description
The EHA105 strain by EHA101 strain and C58 type transformation, as the background, with screening label, rifampicin resistant gene RIF in nuclear genes, in order to facilitate the transformation of the operation, the strains carrying Ti plasmid pEHA105 amber base without its transport function (pTiBo542DT-DNA), the plasmid containing vir gene (VIR T-DNA gene was inserted into the plant genome the essential components of pEHA105 (pTiBo542DT-DNA) plasmid T-DNA transfer function itself is destroyed, but can be transferred to the binary vector T-DNA help smooth transfer). The strain of EHA105 can be grown on YEB or LB medium. The optimum temperature is 28centigrade oxygen consumption, and 30% glycerol can be used to preserve the bacteria. The method of plasmids conversion is chemical conversion and electrical transformation.
EHA105 Agrobacterium Strain Genotype
C58 (rif R) Ti pEHA105 (pTiBo542DT-DNA) Succinamopine
EHA105 Agrobacterium Strain Reference
1.High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes
Tomasz Kowalczyk鈥? Aneta Gerszberg, Paulina Dura艅ska, R贸偶a Bi艂as and Katarzyna Hnatuszko-Konka鈥燛mail authorView ORCID ID profile
鈥燙ontributed equally
AMB Express 20188:125
https://doi.org/10.1186/s13568-018-0656-6漏 The Author(s) 2018
Received: 20 July 2018Accepted: 30 July 2018 Published: 6 August 2018
Caution:
1. This product is FOR RESEARCH USE ONLY!
2.Please kindly culture it as soon as possible then harvest and store at -80鈩?
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Growth and Storage of Agrobacterium
Proper preservation of strains is of great importance to all microbiologists. Working with contaminated or genetically changed cultures, or facing the loss of a strain are annoyances that often may be easily prevented. A reliable preservation
Electroporation of Agrobacterium tumefaciens
Agrobacterium tumefaciens is a soil bacterium that causes tumors on dicotyledonous plants. Virulent strains harbor a large plasmid, the Ti (tumor-inducing) plasmid, which is involved in tumorigenesis. A small segment of this plasmid, the T
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