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- 2025年10月07日
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- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
3个月
- 英文名：
EHA101 Agrobacterium Strain
- 库存：
50
- 供应商：
LSM BIO
- 规格：
100ul
EHA101 Agrobacterium Strain
Packing 100ul
Storage condition -80鈩?/p>
EHA101 Agrobacterium Strain Information
Type Agrobacterium tumefaciens
Resistance: Rif Kan
Culture medium: YEB
Strain category: Agrobacterium tumefaciens
Culture conditions: 30 centigrade, aerobic, LB
Transformation of plasmids: electric conversion and transformation
Preservation method: 30% glycerin, -80鈩?/p>
Basic application: transgene
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. Please kindly culture it as soon as possible then harvest and store at -80鈩?
Related Strain
A4 Agrobacterium Strain
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EHA101 Agrobacterium Strain
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GV3101 Agrobacterium Strain
LBA4404 Agrobacterium Strain
LBA9402 Agrobacterium Strain
P3301/ LBA4404 Agrobacterium Strain
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文献和实验Agrobacterium growth and transformation
. This is not very efficient (~200 colonies per microgram of DNA), but it is really easy.1. Pick a single colony of the Agrobacterium strain of choice and inoculate 3 ml of LB (or 2YT) in a 15 ml snap-cap tube (Falcon tube). Grow @ 30℃ overnight on a roller drum. Be sure
Growth and Storage of Agrobacterium
Proper preservation of strains is of great importance to all microbiologists. Working with contaminated or genetically changed cultures, or facing the loss of a strain are annoyances that often may be easily prevented. A reliable preservation
Electroporation of Agrobacterium tumefaciens
Agrobacterium tumefaciens is a soil bacterium that causes tumors on dicotyledonous plants. Virulent strains harbor a large plasmid, the Ti (tumor-inducing) plasmid, which is involved in tumorigenesis. A small segment of this plasmid, the T
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