Y2HGold 感受态细胞(化转)/ Y2HGold che

Y2HGOLD Chemically Competent Cell

Packing 10 x 0.1 ml/tube

Alternative name
Y2HGOLD chemically Competent Cells,Y2HGOLD Competent E.coli,Y2HGOLD Competent Cells, Y2HGOLD chemically Yeast Express Competent Cells

 

Transformation Protocol

1.Please store Y2HGOLD Chemically Competent Cell in -80°C for late use.

2. Add plasmid DNA to the cell mixture (do not Thaw).

3. Heat shock at exactly 37°C for exactly 5min.Carefully flick the tube 1-2 times .

4. Add 1.5ml Solution 1, MIX.

5. Heat shock at exactly 30°C for exactly 1 hour.

6. Pellet the mixture by centrifugation at 2000rpm/min for 10min, Pour off cell supernatant, then resuspend the cell pellet in the remaining 1.5ml Solution 2.

7. Pellet the mixture by centrifugation, Pour off cell supernatant, then resuspend the cell pellet in the remaining 0.2ml Solution 2.

8. Spread the transformation liquid on the MD plates, then place it on 30°C for 3-4 days.

 

Caution:

1.STORAGE AND HANDLING: Competent cells should be stored at

–80°C. Storage at –20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above –80°C, even if they do not thaw.

2. This product is FOR RESEARCH USE ONLY!