- ￥980
- LSM Bio
- CHC00056
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
6month
- 英文名：
Y2HGold chemically Yeast Express Competent Cells
- 库存：
货源充足
- 供应商：
LSM Bio
- 规格：
0.1ml*10
Y2HGOLD Chemically Competent Cell
Packing 10 x 0.1 ml/tube
Alternative name
Y2HGOLD chemically Competent Cells,Y2HGOLD Competent E.coli,Y2HGOLD Competent Cells, Y2HGOLD chemically Yeast Express Competent Cells
Transformation Protocol
1.Please store Y2HGOLD Chemically Competent Cell in -80°C for late use.
2. Add plasmid DNA to the cell mixture (do not Thaw).
3. Heat shock at exactly 37°C for exactly 5min.Carefully flick the tube 1-2 times .
4. Add 1.5ml Solution 1, MIX.
5. Heat shock at exactly 30°C for exactly 1 hour.
6. Pellet the mixture by centrifugation at 2000rpm/min for 10min, Pour off cell supernatant, then resuspend the cell pellet in the remaining 1.5ml Solution 2.
7. Pellet the mixture by centrifugation, Pour off cell supernatant, then resuspend the cell pellet in the remaining 0.2ml Solution 2.
8. Spread the transformation liquid on the MD plates, then place it on 30°C for 3-4 days.
Caution:
1.STORAGE AND HANDLING: Competent cells should be stored at
–80°C. Storage at –20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above –80°C, even if they do not thaw.
2. This product is FOR RESEARCH USE ONLY!
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文献和实验a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
J-6B centrifuge)for 10 min.at 4℃.4度2500g离心10分钟。5、Resuspend cells gently in 80 ml of ice cold "TB".小心用80ml预冷TB重悬细胞。6、On ice for 10 minutes.(30min)菌液置冰上10分钟。7、Spin at 2500 x g (5000 rpm in a Sorvall GSA,5500 rpm in a Sorvall SS-34,or 3000 rpm
! pH 6.7 - 7.0 Note: Competent Cell s are fragile (cell wall is thought to be weakened),therefore treat the cells gently when preparing these cells.Do not vortex or pippette up and down to resuspend the cells.Do not spin
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