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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负80摄氏度
- 保质期:
6month
- 英文名:
Stable Electro Competent Cells
- 库存:
货源充足
- 供应商:
LSM Bio
- 规格:
0.5ml*10
Stable Electro Competent Cells
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文献和实验Establishment of Stable Transfectant of CHO Lec Cells
Cells should be 50~70% confluent. Detach cells with trypsin/EDTA, wash once w/ complete medium, and count cell number.Suspend cells in EPBS at ~1 x 107/ml (Typical yield is ~2 x 107 cells/flask)Add 800 µl of cells to cuvette plus DNA (dissolved
Human Pregnane X Receptor (hPXR) Activation Assay in Stable Cell Lines
Analysis of pregnane X receptor (PXR, NR1I2) activation to determine induction of drug metabolizing enzymes and transporters and predict drug-drug interactions (DDIs) is a wildly used technique among in vitro assays. Direct assessment of PXR
Stable Transfection (Electroporation)
Stable Transfection (Electroporation) Outline: Electroporation can be used for both transient and stable transfection of mammalian cells. Cells are placed in suspension in an appropriate electroporation buffer
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