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- 15250061
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- 2025年08月08日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
15 – 30°C
- 规格：
15250061
描述
Trypan Blue Solution, 0.4%, is routinely used as a cell stain to assess cell viability using the dye exclusion test. This test is often performed while counting cells with the hemocytometer during routine subculturing, but can be performed any time cell viability needs to be determined quickly and accurately. The dye exclusion test is based upon the concept that viable cells do not take up impermeable dyes (like Trypan Blue), but dead cells are permeable and take up the dye.cGMP manufacturing and quality system
Trypan Blue Solution is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.
规格
| Color: | Blue |
|---|---|
| For Use With (Equipment): | Light Microscope |
| Sub-Cellular Localization: | Cytoplasm & Cytosol |
| Detection Method: | Colorimetric |
| Label or Dye: | Trypan Blue |
| Product Size: | 100 mL |
| Green Features: | Sustainable packaging |
| Shipping Condition: | Room Temperature |
内容及储存
Storage conditions: 15 – 30°CShipping conditions: Ambient
Shelf life: 36 months from date of manufacture
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- 作者
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文献和实验and resuspended in protein-free medium or salt solution prior to counting.Supplies & Equipment:Eppendorf tubes (1.5 ml)Micropipet (10µl)HemocytometerReagents:HBSS (Hanks' Balanced Salt Solution)sterile Trypan blue solution 0.4% (Sigma T-8154)Procedure:Prepare
(Hanks' Balanced Salt Solution) sterile Trypan blue solution 0.4% (Sigma T-8154) Procedure: Prepare a cell suspension in HBSS Transfer into Eppendorf tube: 0.5 ml of 0.4% Trypan blue solution 0.3 ml
Protocol Protein Gel Staining Using Coomassie Blue Protein Stain
Materials Polyacrylamide pre-cast gel SimplyBlue™ Coomassie protein stain, 1x pre-mixed solution (Coomassie blue dye binds to proteins (arginine, the aromatic amino acids, and histidine) allowing protein bands to be visualized
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