Target Protein Name:Human Secretory carrier-associated membrane protein 1
Alternative Name:Secretory carrier-associated membrane protein 1, Secretory carrier membrane protein 1, SCAMP1, SCAMP Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human SCAMP1 ELISA Kit allows for the in vitro quantitative determination of Human Secretory carrier-associated membrane protein 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human SCAMP1 ELISA Kit has been pre-coated with an SCAMP1 antibody specific to Human Secretory carrier-associated membrane protein 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Secretory carrier-associated membrane protein 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Secretory carrier-associated membrane protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Secretory carrier-associated membrane protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!