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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human HRP 2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
HRP 2 ELISA KIT|Human
Catalog EF009601
Packing 48T/96T
Reactivity Human
Detect Range 0.313-20ng/ml
HRP 2 ELISA KIT Sensitivity< 0.188ng/ml
Application HRP 2 ELISA KIT|Human is for detecting the concentration of Human HRP 2 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of HRP 2 ELISA KIT|Human
The microtiter plate provided in HRP 2 ELISA KIT|Human has been pre-coated with an Human HRP 2 antibody specific to Human HRP 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human HRP 2antibody preparation specific for Human HRP 2 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human HRP 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human HRP 2in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that HRP 2 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验人 Neuritin 酶联免疫分析(ELISA) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本 中Neuritin 的含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中人 Neuritin 水平。用纯化的 Neuritin 抗体 包被微孔板,制成固相抗体,往包被单抗的微孔中加入 Neuritin ,再与 HRP 标记的 Neuritin 抗体结合,形成抗体
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
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