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- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human ALP
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1NG/ML
- 规格:
96T
ALP ELISA KIT|Human
Catalog EF009206
Packing 48T/96T
Reactivity Human
Detect Range 3.125-200ng/ml
ALP ELISA KIT Sensitivity< 1.875ng/ml
Application ALP ELISA KIT|Human is for detecting the concentration of Human ALP in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of ALP ELISA KIT|Human
The microtiter plate provided in ALP ELISA KIT|Human has been pre-coated with an Human ALP antibody specific to Human ALP . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human ALP antibody preparation specific for Human ALP then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human ALP , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human ALP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that ALP ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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