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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Cytokeratin 15
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
Cytokeratin 15 ELISA KIT|Human
Catalog EF008987
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
Cytokeratin 15 ELISA KIT Sensitivity< 0.094ng/ml
Application Cytokeratin 15 ELISA KIT|Human is for detecting the concentration of Human Cytokeratin 15 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of Cytokeratin 15 ELISA KIT|Human
The microtiter plate provided in Cytokeratin 15 ELISA KIT|Human has been pre-coated with an Human Cytokeratin 15 antibody specific to Human Cytokeratin 15. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human Cytokeratin 15antibody preparation specific for Human Cytokeratin 15 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Cytokeratin 15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human Cytokeratin 15in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that Cytokeratin 15 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验samples, MPC-15 for 1–15 ml samples and MPC-50 for 15–50 ml samples. Mixer allowing both tilting and rotation. Buffer 1: PBS (without Ca2 and Mg2 ) w/0.1% BSA and 2 mM EDTA, pH 7.4. Buffer 2: PBS pH 7.4 (without Ca2 and Mg2 ).
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
, and Ki-67 antibodies), while the staining of many other antibodies may be enhanced by epitope retrieval protocol (e.g, S-100, cytokeratin, and synaptophysin antibodies). You may use any other epitope retrieval protocol recommended by the supplier
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