- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 国产
- EF008979
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Cystatin F
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
Cystatin F ELISA KIT|Human
Catalog EF008979
Packing 48T/96T
Reactivity Human
Detect Range 0.313-20ng/ml
Cystatin F ELISA KIT Sensitivity< 0.188ng/ml
Application Cystatin F ELISA KIT|Human is for detecting the concentration of Human Cystatin F in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of Cystatin F ELISA KIT|Human
The microtiter plate provided in Cystatin F ELISA KIT|Human has been pre-coated with an Human Cystatin F antibody specific to Human Cystatin F. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human Cystatin Fantibody preparation specific for Human Cystatin F then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Cystatin F, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human Cystatin Fin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that Cystatin F ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
实验试剂 1. CytoTune™ Sendai Reprogramming Vectors Note: For successful reprogramming, you need all four reprogramming vectors. 2. Human neonatal foreskin fibroblast cells to reprogram 3. Gibco® Mouse
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
的干扰。最好用亲和层析纯的抗体，这样全部酶结合物均具有特异的免疫活性，可以在高稀释度进行反应，实验结果本底浅淡。如用F(ab')2进行标记，则更可避免标本中RF的干扰。在ELISA中用酶标抗原的模式不多，总的要求是抗原必须是高纯度的。 （三）结合物的制备 酶标记抗体的制备方法主要有两种，即戊二醛交联法和过碘酸盐氧化法。 （1）戊二醛交联法：戊二醛是一种双功能团试剂，它可以使酶与蛋白质的氨基通过它而联结。碱性磷酸一般用此法进行标记。交联方法一步法、两步法两种。在一步法中戊二醛直接加入酶与抗体
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