- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 国产
- EF008383
- 2025年07月02日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Carboxypeptidase A3
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
Carboxypeptidase A3 ELISA KIT|Human
Catalog EF008383
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
Carboxypeptidase A3 ELISA KIT Sensitivity< 0.094ng/ml
Application Carboxypeptidase A3 ELISA KIT|Human is for detecting the concentration of Human Carboxypeptidase A3 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of Carboxypeptidase A3 ELISA KIT|Human
The microtiter plate provided in Carboxypeptidase A3 ELISA KIT|Human has been pre-coated with an Human Carboxypeptidase A3 antibody specific to Human Carboxypeptidase A3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human Carboxypeptidase A3antibody preparation specific for Human Carboxypeptidase A3 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Carboxypeptidase A3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human Carboxypeptidase A3in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that Carboxypeptidase A3 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Analysis of MCT and MCTC Mast Cells in Tissue
The MCTC and MCT types of human mast cells initially were recognized on the basis of the protease compositions of their secretory granules, with tryptase, chymase, carboxypeptidase A3, and cathepsin G in the former and only tryptase
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
的原理用于分子诊断方面：TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的，相关文章见：1，Rapid screen for truncating ATM mutations by PTT-ELISA . 2，An ELISA-based high throughput protein truncation test
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