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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human alpha Adaptin
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
alpha Adaptin ELISA KIT|Human
Catalog EF007725
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
alpha Adaptin ELISA KIT Sensitivity< 0.094ng/ml
Application alpha Adaptin ELISA KIT|Human is for detecting the concentration of Human alpha Adaptin in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of alpha Adaptin ELISA KIT|Human
The microtiter plate provided in alpha Adaptin ELISA KIT|Human has been pre-coated with an Human alpha Adaptin antibody specific to Human alpha Adaptin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human alpha Adaptinantibody preparation specific for Human alpha Adaptin then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human alpha Adaptin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human alpha Adaptinin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that alpha Adaptin ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
markers, specific for type I cells, have been described in the recent past. The first one was T1α, a 36-kDa glycoprotein found in rodent lungs. Antibodies have been developed against lung proteins that specifically label type I cells in human lung
一篇文献中的ELISA方法如下:Control peptides (14–17 amino acid sequence near the N-terminal or C-terminal of the studied protein) and the reactive rabbit anti-defensin antibodies (affinity pure) were obtained from Alpha Diagnostics .CoStar flat-bottom high
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