- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 国产(香港)
- EF007351
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human IL-12 p35
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
IL-12 p35 ELISA KIT|Human
Catalog EF007351
Packing 48T/96T
Reactivity Human
Detect Range 31.25-2000pg/ml
IL-12 p35 ELISA KIT Sensitivity< 18.75pg/ml
Application IL-12 p35 ELISA KIT|Human is for detecting the concentration of Human IL-12 p35 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of IL-12 p35 ELISA KIT|Human
The microtiter plate provided in IL-12 p35 ELISA KIT|Human has been pre-coated with an Human IL-12 p35 antibody specific to Human IL-12 p35. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human IL-12 p35antibody preparation specific for Human IL-12 p35 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human IL-12 p35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human IL-12 p35in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that IL-12 p35 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验来说，IL-12p35是其限制因素，是调控免疫反应的主要因素。IL-12p70是启动Th1反应的关键细胞因子，DC在成熟的晚期和向T细胞递呈抗原的早期，DC可分泌具有生物活性的IL-12p70，IL-12p70可诱导Th0向Th1分化，启动细胞免疫，决定免疫激活还是免疫耐受。因此，IL-12p70在抗肿瘤作用和免疫激活中发挥不可替代的作用。丁香园网友cj_1968认为：不知mapan777做IL-12的PCR是什么目的？如果检测IL-12的表达应该做ELISA，如果想重组IL-12p70我个人认为有相当
) abs510003-96T Human IL-6 ELISA Kit abs510006-96T Human TNF-α ELISA Kit abs510002-96T Human IL-1β ELISA Kit abs510004-96T Human IL-8/CXCL8 ELISA Kit abs520003-96T Mouse IL-4 ELISA Kit abs520006-96T Mouse IL-12 p70 ELISA Kit abs520007-96
-HS Human IL-10 ELISA Kit 0.469pg/ml 0.781-50pg/ml (1ng=14500mIU) EH4146 Human IL-12 (p35) ELISA Kit 18.75pg/ml 31.25-2000pg/ml EH0176 Human IL-12 ELISA Kit 9.375pg/ml 15.625-1000pg/ml (1pg=11mIU
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